Fig. 1.

Terminal-Tem are a distinct subset of CD8 T cells contained within the conventional Tem population. P14 CD8 T cells were adoptively transferred into congenically distinct recipient mice that were subsequently infected with LCMV. (A) Expression of KLRG1, CD127 (Left), and CD27 (Right) by Tcm (CD62L^hi) and conventional (Conv.) Tem (CD62L^lo) within peripheral blood lymphocytes (PBLs) on day 70 of infection. (B) Expression of indicated molecules on P14 PBLs in response to LCMV infection. Frequency of CD127^loCD62L^lo (highlighted red), CD127^hiCD62L^lo (highlighted gray), and CD127^hiCD62L^hi (highlighted blue) in PBLs following LCMV infection (Bottom). (C) Representative expression patterns of indicated molecules by terminal-Tem, Tem, Conv. Tem, and Tcm. (D) Representative flow cytometry plots demonstrating the frequency of terminal-Tem, Tem, and Tcm in indicated tissues (>30 d postinfection). Splenic red and white pulp localized P14 cells were discriminated by intravascular staining of CD8α. (E) Principal component analysis of the RNA-seq transcriptional profile of splenic terminal-Tem, Tem, Conv. Tem, and Tcm P14 cells on day 55 of infection (Left), and heatmap illustrating differentially expressed genes (≥1.5-fold) ordered through k-means clustering (Top Right) among terminal-Tem, Tem, Conv. Tem, and Tcm, or highlighted key genes (Bottom). Numbers in plots are the frequency of cells in the indicated gate (A–D). All data are from two independent experiments with n = 3 to 5 per timepoint, and RNA-seq samples consist of two biological replicates wherein each replicate is comprised of cells pooled from two mice. Graphs indicate mean ± SEM, and symbols represent an individual mouse (B).