Table 1.
ADAM17 cleavage sites in protein substrates reported and identified by SPD-NGS
| Gene | Cleavage site | PSSM score | Distance to membrane (no. of amino acids) |
| TNF | LAQAVRSS | 11.56 | 11 |
| TGFA | DLLAVVAA | 8.6 | 9 |
| TNFR | PAEGSTGD | 1.73 | 4 |
| IL1R2 | EASSTFSW | 1.3 | −3 |
| COL17A1 | EKDRLQGM | 2.26 | 35 |
| TFRC | ECERLAGT | 5.5 | 18 |
| IL6R | TSLPVQDS | 5 | 7 |
| NOTCH1 | KIEAVQSE | 4.2 | 15 |
| PTPRZ1 | LAEGLESE | 10.4 | 6 |
| APP | HHQKLVFF | <0 | 14 |
| PMEL | VSTQLIMP | 1.5 | 12 |
| VASN | TPPAVHSN | 2.21 | 17 |
| CD44 | SHGSQEGG | 0.6 | 19 |
| BTC | DLFYLQQD | 1.8 | 7 |
| ERBB4 | STLPQHAR | 5 | 6 |
| ACE | NSARSEGP | 3.2 | 24 |
| TNFRSF1B | FALPVGLI | 1.02 | 0 |
Reported cleavage sites are shown for proteins established as ADAM17 substrates through genetic, knockdown, or proteomics approaches. The same cleavage events were identified in SPD-NGS with PSSM scores, and their distances to membrane are listed. The distance to membrane was obtained from UniProtKB topological domain information.