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. 2020 Oct 17;18:144. doi: 10.1186/s12951-020-00707-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effect of serum and heparin sodium on the stability of LOrn1/DNA lipoplexes with a charge ratio of 4:1 and LOrn3/DNA lipoplexes with an charge ratio of 3:1. a Agarose gel electrophoresis of the lipoplexes at different serum contents (Lane M: marker; Lane DNA: free DNA; Lanes 1–6: serum contents of 10%, 15%, 20%, 25%, 30%, and 40%, respectively). b Quantitative analysis of the dissociation rate of lipoplexes in the present of serum. c Agarose gel electrophoresis of the lipoplexes at different concentrations of heparin sodium (Lane M: marker; Lane DNA: free DNA; Lanes 1–6: heparin sodium concentrations of 0.05, 0.1, 0.5, 1.0, 1.5, and 2.0 µg/µL, respectively). d Quantitative analysis of the dissociation rate of lipoplexes in the present of heparin sodium. Each bar represents the mean ± SD (n = 5). Significance levels: **p < 0.01