Fig. 3.

miR-27a-3p was the target of LINC01089 in GC. a WT-LINC01089 and MUT-LINC01089 luciferase reporter vectors containing the binding site for miR-27a-3p were constructed. b miR-27a-3p mimics or control miRNA were co-transfected with WT-LINC01089 or MUT-LINC01089 into 293T cells, and then the luciferase activity of each group was measured. c RIP assay confirmed that LINC01089 and miR-27a-3p were directly interacted in the RISC complex. d qRT-PCR was utilized to investigate the expression level of miR-27a-3p in paired tumor tissues and adjacent non-tumor tissues. (NT, non-tumor tissues). e Pearson’s correlation analysis indicated that LINC01089 and miR-27a-3p expression levels were negatively correlated in GC tissues. ** symbolizes P < 0.01, and *** symbolizes P < 0.001