Fig. 5.

TET1 was a downstream target of miR-27a-3p in GC. a WT-TET1 3′UTR and MUT-TET1 3′UTR luciferase reporter vectors containing the binding site for miR-27a-3p were constructed. b miR-27a-3p mimics were co-transfected with WT-TET1 or MUT-TET1 into 293T cells. Then the luciferase activity of each group was measured by the double luciferase assay. c Pearson’s correlation analysis demonstrated that miR-27a-3p and TET1 expression levels were negatively correlated in GC tissues. d Pearson’s correlation analysis demonstrated that LINC01089 and TET1 expression levels were positively correlated in GC tissues. e miR-27a-3p mimics were transfected into MGC-803 cells with LINC01089 overexpression, and anti-miR-27a-3p was transfected into AGS cells with LINC01089 knockdown. Then qRT-PCR was utilized to detect the expression level of miR-27a-3p in GC cells. f, g Western blot was utilized to investigate the expression of TET1 protein in MGC-803 cells and AGS cells after transfection. ** symbolizes P < 0.01, and *** symbolizes P < 0.001