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. 2020 Oct 17;20:315. doi: 10.1186/s12866-020-02010-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The procedure and interpretation of mCIM and eCIM. a. A 1-μL loopful of test CRE isolate is resuspended in two tubes containing 2 mL of TSB. One tube is devoid of EDTA (mCIM), while the other is supplemented with EDTA (eCIM). A meropenem (MEM) disk is submerged in each tube, and the tubes are incubated at 35 °C for 4 h ± 15 min. The disks are then removed from the tubes and placed on MH agar plates upon which a carbapenem-susceptible reporter E. coli ATCC 25922 has been freshly applied. The plates are incubated at 35 °C for 16 to 20 h before the zone sizes are recorded. b. Interpretation of mCIM and eCIM tests of 4 clinical K. pneumoniae isolates. Isolate 459 was carbapenemase negative; 429 had metallo-β-lactamase (bla_NDM-5); 448 had serine carbapenemase (bla_OXA-48); 451 had serine carbapenemase (bla_KPC-2)