Fig. 7.

MIR100HG knockdown inhibits tumour growth by upregulating miR-5590-3p in a xenograft mouse model MDA-MB-231 cell xenograft tumours stably transfected with MIR100HG shRNA or shRNA Ctrl vectors were obtained. The sizes of the tumours was then recorded. a, b Periodic monitoring of tumour dimensions after MIR100HG knockdown. c Tumour weights on day 28. d The expression levels of MIR100HG, miR-5590-3p and OTX1 in tumour tissues on day 28. e The protein levels of OTX1 in tumour tissues, as assessed by IHC assays. Scale bar: 50 µm. f, g The protein levels in the ERK/MAPK signalling pathway and the OTX1 protein in tumour tissues, as assessed by western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.001