Table 5.

Effect of protease and soybean meal source¹ on small intestinal histology and permeability to FITC dextran, and electrophysiological properties²

	Without protease		With protease3			P-value
Item	SBM 1	SBM 2	SBM 1	SBM 2	SEM	SBM source	Protease	SBM source × protease
Jejunal histology
Villus height, µm	393.0	394.5	407.7	402.8	16.45	0.920	0.499	0.851
Crypt depth, µm	199.1	200.3	206.1	211.6	6.15	0.596	0.155	0.734
VH:CD4	1.97	1.99	1.99	1.90	0.074	0.662	0.639	0.484
FITC dextran flow, mg/h
Jejunum	0.138	0.114	0.068	0.101	0.024	0.841	0.099	0.251
Ileum	0.124	0.089	0.116	0.108	0.031	0.486	0.871	0.669
Resistance, Ω
Jejunum	72.67	66.81	72.84	84.32	10.82	0.797	0.421	0.430
Ileum	82.38	81.54	96.12	101.90	12.96	0.850	0.199	0.800
Basal Isc, μA/cm2
Jejunum	−0.13	0.00	0.01	−0.01	0.030	0.304	0.223	0.297
Ileum	−0.02	−0.06	−0.01	0.02	0.031	0.403	0.352	0.659
SGLT1 dependent Isc, μA/cm2
Jejunum	0.11	0.11	0.18	0.12	0.192	0.498	0.238	0.538
Ileum	0.13	0.17	0.11	0.12	0.095	0.828	0.412	0.263

¹SBM 1, soybean meal from Volga, SD; SBM 2, soybean meal from Beresford, SD.

²Data are means of 12 observations.

³Protease (ProAct, DSM Nutritional Products) supplied 15,000 units of protease per kilogram of diet.

⁴VH:CD, villus height to crypt depth ratio.