Skip to main content
. Author manuscript; available in PMC: 2020 Oct 17.
Published in final edited form as: Methods Mol Biol. 2019;2042:151–164. doi: 10.1007/978-1-4939-9694-0_11

Figure 1-.

Figure 1-

Schematic of overall cloning strategy used for constructing allelic exchange plasmid. (1) The 3 kb left flank (upstream) of gene of interest (ct00X) is PCR-amplified from C. trachomatis L2 genomic DNA and cloned into linearized (SalI) pSuMc-sal-sbf by Gibson Assembly to yield pSUmC-sal-sbf + LF. (2) The 3 kb right flank (downstream) of gene of interest (ctl00X) is then mobilized into linearized (SalI) pSuMc-sal-sbf + LF ct00X to yield the final construct pSU-Δ00X.