Table 3.
Detection of SARS-CoV-2 RNA from raw sewage.
| Date | Plant | Concentration factor | log10 Wa | SLOQ (copies/mL) |
SARS-CoV-2 |
|||
|---|---|---|---|---|---|---|---|---|
| CDC N1 |
CDC N2 |
CDC N3 |
NIID | |||||
| June 30 | D | 160 | −0.41 | 16.1 | Neg | Neg | Neg | Neg |
| E | 149 | −0.76 | 38.5 | Neg | Neg | Neg | Neg | |
| July 7 | D | 152 | −0.79 | 40.6 | Posb | Neg | Neg | Neg |
| E | 163 | −0.65 | 27.5 | Neg | Neg | Neg | Neg | |
| July 16 | D | 152 | 0.04 | 6.0 | Neg | Neg | Posc | Neg |
| E | 146 | −0.29 | 13.2 | Neg | Neg | Neg | Neg | |
| July 22 | D | 159 | −1.14 | 86.6 | Neg | Neg | Neg | Neg |
| E | 160 | −0.34 | 13.5 | Neg | Neg | Neg | Neg | |
| July 29 | D | 159 | −0.36 | 14.3 | Posd | Neg | Neg | Neg |
| E | 160 | −0.26 | 11.3 | Pose | Neg | Neg | Neg | |
| August 5 | D | 154 | −0.2 | 10.4 | Neg | Neg | Neg | Neg |
| E | 154 | −0.68 | 31.3 | Neg | Neg | Neg | Neg | |
Neg: negative, Pos: positive.
Whole process recovery ratio (W) of φ6 was determined at n = 1 for each sample.
SARS-CoV-2 CDC N1 gene was positive; the Ct was 38.7 (in one of two PCR reactions).
SARS-CoV-2 CDC N3 gene was positive; Ct was 38.9 (in one of two PCR reactions).
SARS-CoV-2 CDC N1 gene was positive; the Ct was 40.9 (in one of two PCR reactions).
SARS-CoV-2 CDC N1 gene was positive; the Ct was 37.7 (in one of two PCR reactions).