Figure 2.

Generation of FD-KO mice. (a) Scheme of gene editing for deficiency of Factor D gene by cloning free CRISPR/Cas9 system. Exons are showed by gray boxes and introns are indicated by gray lines. The sequences in squares are target of crRNA. PAM sequences are showed by underline. (b) Genotyping of FD-KO mice. 2462-bp band shows wild-type allele, and 531-bp band shows knockout allele. M shows molecular weight marker. This image was cropped and the full image was shown in Supplementary Fig. 2. (c) Relative mRNA expression levels of Factor D in the adrenal gland and subcutaneous adipose tissue of WT or FD-KO mice. N = 4 in each group. Data are shown as mean ± SEM. *p < 0.05, ***p < 0.001 by an unpaired two-tailed t test. (d) Body weight and food intake, (e) weight of liver or epididymal adipose tissue, (f) glucose and insulin level in blood of WT or FD-KO mice. N = 7, 6, 7, 9. *p < 0.05, ** p < 0.01, ****p < 0.0001 by one-way ANOVA followed by a Tukey's post-hoc test.