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. 2020 Oct 16;11(10):871. doi: 10.1038/s41419-020-03080-x

Fig. 2. Relative expression of PVT1 in GBC cell lines and its effect on the proliferation of GBC cells.

Fig. 2

A Relative expression of PVT1 in GBC cell lines and human biliary epithelium cell line H69 determined by qRT-PCR. B The efficiency of siRNAs of PVT1 in GBC-SD cells examined by qRT-PCR. C The efficiency of pcDNA-PVT1 in SGC-996 cells examined by qRT-PCR. D The proliferation ability of GBC-SD cells transfected with si-PVT1-1 and SGC-996 cells transfected with PVT1 plasmid determined by CCK8 assays. E The cloning ability of transfected GBC-SD and SGC-996 cells. F The effect of PVT1 on cell cycle of GBC-SD and SGC-996 cells. G The effect of PVT1 on the expression of CDK4 and CyclinD1 in GBC cells examined by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001.