TABLE 1.
Antigens included in the bacteriophage MS2 virus-like particle C. trachomatis antigen fragment library for DSCB and most commonly selected epitope(s) from DSCB
|
C. trachomatis
|
Epitope(s) most selected | Amino acid position(s) | No. of individual selections | No. of patients selecting | Infection outcomea |
|||
|---|---|---|---|---|---|---|---|---|
| Antigen | No. | SR | NR | R | ||||
| Major outer membrane protein (MOMP) | CT681 | EFTINKPKGYVGKEFPLD | 233–250 | 80 | 26/30 | 7 | 9 | 10 |
| FDTTTLNPTIAGAGDV | 314–329 | 21/30 | 6 | 10 | 5 | |||
| 60-kDa cysteine-rich OMP (OmcB) | CT443 | ATGPKQDSCFGRMY | 86–99 | 34 | 19/30 | 6 | 7 | 6 |
| Tarp | CT456 | NIYESIGGSRTSGPEN | 171–184, 219–234, 271–284b | 30 | 14/30 | 3 | 6 | 5 |
| Solute protein-binding family (metal ion transport protein) | CT067 | MNSCSSSRGNQPADESIY | 25–42 | 28 | 17/30 | 7 | 6 | 4 |
| Putative outer membrane protein C (PmpC) | CT414 | LEGSQGDTADTGT | 647–659 | 24 | 11/30 | 5 | 5 | 1 |
| Hypothetical protein | CT147 | WHNQYQM | 1423–1429 | 17 | 15/30 | 7 | 2 | 6 |
| CT875 | CT875 | DPLGRRTPNYQSKNP | 229–243 | 17 | 11/30 | 3 | 3 | 5 |
| Putative outer membrane protein D (PmpD) | CT812 | ASEDGDLSPE | 764–773 | 15 | 10/30 | 4 | 4 | 2 |
| Hypothetical protein pCHL1p5 (plasmid) (Pgp3) | pCT03 | RTSITNTGLT | 208–217 | 14 | 9/30 | 5 | 2 | 2 |
| Inclusion membrane protein A | CT119 | ESSDLCSQIR | 247–256 | 13 | 10/30 | 4 | 2 | 4 |
| Hypothetical protein | CT143 | QAYNCATHRNG | 123–133 | 13 | 9/30 | 3 | 4 | 2 |
| L31 ribosomal protein | CT022 | CGSTYQTDKT | 25–34 | 12 | 11/30 | 5 | 3 | 3 |
| CHLPN 76-kDa homolog | CT622 | LKQEHTGLTD | 283–292 | 12 | 9/30 | 5 | 2 | 2 |
| Hypothetical protein | CT695 | RTTSSSGVSED | 57–67 | 11 | 9/30 | 3 | 3 | 3 |
| Aspartyl tRNA synthetase | CT542 | NQALDHLRRL | 403–412 | 11 | 9/30 | 4 | 3 | 2 |
| Hypothetical protein | CT795 | LIYPPV | 105–110 | 9 | 9/30 | 3 | 1 | 5 |
| CQQEAEEDC | 27–35 | 7/30 | 5 | 1 | 1 | |||
| Arginine binding protein (ArtJ) | CT381 | TGCLKEGGDS | 22–31 | 9 | 9/30 | 4 | 3 | 2 |
| HSP60 | CT110 | EDEQIGARIV | 436–445 | 9 | 7/30 | 4 | 1 | 2 |
| Hypothetical protein | CT101 | SMLKQHELDA | 65–74 | 8 | 8/30 | 2 | 4 | 2 |
| Thio-specific antioxidant (TSA) peroxidase | CT603 | VINDLPLGRSI | 138–149 | 8 | 7/30 | 2 | 2 | 3 |
| Recombination protein | CT240 | ISQTSPCNF | 66–74 | 8 | 7/30 | 5 | 2 | 0 |
| SPITGKHLS | 114–122 | 7/30 | 2 | 2 | 3 | |||
| DO serine protease | CT823 | LNQVLKNSKGENV | 466–477 | 8 | 6/30 | 3 | 1 | 2 |
| ATP-dependent zinc metalloprotease | CT841 | LSKEVRNAIT | 118–127 | 7 | 7/30 | 3 | 4 | 0 |
| PGRFDRRV | 584–591 | 7/30 | 3 | 3 | 1 | |||
| FKBP-type peptidyl-prolyl cis-trans isomerase | CT541 | ALTDNQKLS | 40–48 | 5 | 5/30 | 2 | 1 | 2 |
| IKGMQSEI | 73–80 | 5/30 | 2 | 1 | 2 | |||
| GMQGMKEGE | 193–201 | 3/30 | 2 | 0 | 1 | |||
a
SR, spontaneous resolver; NR, nonreinfected; R, reinfected.
b
Multiple amino acid positions are indicated for a single peptide sequence because this is a repeated sequence in the Tarp antigen.