TABLE 2.
Mating-out assays using blaKPC-2 donors into several recipientsa
| Donor strain |
Recipient name |
Recipient species |
Recipient ST |
Recipient origin |
Recipient isolation site |
Transconjugant name |
Frequency | MEM MIC change (R/TC) |
|---|---|---|---|---|---|---|---|---|
| P16 | J53 | E. coli | 10 | Lab strain | P16-KPC-TC | 5 × 10−4 | 3 log2 dilutions (≤0.03/0.25) |
|
| P16-KPC-TC | P52 | K. pneumoniae | 258 | Brazil | Human blood |
P52-TC | 1.13 × 10−6 | 4 log2 dilutions (2/32) |
| P16-KPC-TC | HSP65 | K. pneumoniae | 101 | Brazil | Human blood |
HSP65-TC | 3 × 10−6 | 3 log2 dilutions (1/8) |
| P16-KPC-TC | HSP32 | K. pneumoniae | 15 | Brazil | Human blood |
HSP32-TC | 8.96 × 10−8 | 5 log2 dilutions (≤0.03/1) |
| P16-KPC-TC | 78623 | K. pneumoniae | 185 | Pakistan | Human carriage |
78623-TC | 1.62 × 10−7 | 5 log2 dilutions (≤0.03/1) |
| P16-KPC-TC | 45 | K. pneumoniae | 43 (SLV) | India | Human carriage |
45-TC | 3.5 × 10−7 | 9 log2 dilutions (≤0.03/16) |
| P16-KPC-TC | 22 | K. pneumoniae | 858 (SLV) | India | Human carriage |
|||
| P16-KPC-TC | 4W | K. pneumoniae | 35 | UK | Human carriage |
4W-TC | 3.94 × 10−8 | 4 log2 dilutions (≤0.03/0.5) |
Abbreviations: ST, sequence type; SLV, single locus variant; MEM, meropenem; R, recipient; TC, transconjugant.