TABLE 2.
Hits overrepresented in melarsoprol survivors (comparison of GoF_L2 vs. MEL1)a
| Gene ID | Description (Tryptag 062920) | Localization (TrypTag or proteome) | PubMed | Category | Fold change overrepresented | DESeq P-adjusted value |
|---|---|---|---|---|---|---|
| Tb927.4.4810 | Hypothetical protein, conserved | Cytoplasm (points, weak), endocytic | Endocytic+ | 597 | 3.77E-42 | |
| Tb927.11.590 | Hypothetical protein, conserved | Strong mitochondrial signal | Mitochondrial* | 350 | 1.81E-39 | |
| Tb927.7.2780 | Hypothetical protein, conserved, XAC1 | Cytoplasm | 26784394 | Gene Expression# | 322 | 2.83E-86 |
| Tb927.10.12370 | Gamma-glutamylcysteine synthetase, GSH1 | Nucleoplasm | 8663359 | Biosynthetic# | 191 | 2.19E-43 |
| Tb927.5.3450 | Eukaryotic translation initiation factor eIF2Â | Strong cytoplasm signal | 24945722 | Gene Expression# | 126 | 4.50E-25 |
| Tb927.11.11435 | Dynein light chain lc6 | Strong flagellum axoneme signal | Flagellar+ | 120 | 1.30E-26 | |
| Tb927.7.6190 | Ring finger domain containing protein, ubiqutin ligasê | Strong endocytic system signal | Endocytic+ | 113 | 3.13E-40 | |
| Tb927.11.12380 | Hypothetical protein, conserved | Nuclear localization (mass spectrometry) | Nuclear+ | 97 | 5.89E-48 | |
| Tb927.6.1280 | Translation initiation factor EIF-2b alpha subunit̂ | Cytoplasm | Gene Expression# | 81 | 1.14E-48 | |
| Tb927.8.3820 | Stress granule protein | Localization to starvation stress granules | 26187993 | Stress Granule# | 53 | 2.04E-39 |
| Tb927.3.5250 | Zinc finger CCCH domain-containing protein 8, ZC3H8 | Cytoplasm (points) | 26784394 | Gene Expression# | 53 | 2.45E-13 |
| Tb927.9.10930 | Mediator of RNA polymerase II transcription subunit 7, MED-T7 | Nucleoplasm (points) | 20876299 | Gene Expression# | 49 | 1.98E-18 |
| Tb927.7.770 | Ring finger domain containing protein̂ | Moderate nuclear lumen signal, weak cytoplasm signal | Nuclear+ | 47 | 7.14E-27 | |
| Tb927.9.7820 | Hypothetical protein, conserved | Nuclear, mass spec | Nuclear○ | 39 | 3.88E-14 | |
| Tb927.11.15280 | tRNA-specific adenosine deaminase, ADAT3 | Cytoplasm (reticulated, weak) | 17483465 | Gene Expression# | 37 | 1.59E-18 |
| Tb927.4.1910 | Hypothetical protein, conserved | Cytoplasm (points, reticulated) | 26784394 | Gene Expression# | 35 | 1.10E-22 |
| Tb927.9.15020 | Hypothetical protein, conserved | Weak axoneme signal | Flagellar+ | 31 | 4.45E-20 | |
| Tb927.10.2830 | Hypothetical protein, conserved | Endocytic, cytoplasm | Endocytic+ | 31 | 9.17E-31 | |
| Tb927.10.12050 | LSU ribosomal protein, mitochondrial̂ | Kinetoplast, mitochondrion | 18364347 | Mitochondrial* | 31 | 2.16E-34 |
| Tb927.9.7200 | Hypothetical protein, conserved | Kinetoplast, mitochondrion | Mitochondrial* | 31 | 5.61E-28 | |
| Tb927.7.5460 | Exosome-associated protein 3,3' exoribonuclease, putative, EAP3 | Nucleoplasm | Nuclear+ | 28 | 9.55E-27 | |
| Tb927.4.4540 | Zinc finger domain, LSD1 subclasŝ | Cytoplasm (reticulated) | Zinc finger | 25 | 8.84E-47 | |
| Tb927.5.4370 | Hypothetical protein, conserved | Endocytic, cytoplasm | Endocytic+ | 24 | 3.59E-12 | |
| Tb927.9.7080 | Hypothetical protein, conserved | Cytoplasm (patchy, points) | Mitochondrial & ER○ | 23 | 1.14E-23 | |
| Tb927.2.5210 | 3-Oxoacyl-ACP reductasê | Mitochondrion, kinetoplast (strong) | 17166831 | Mitochondrial# | 23 | 2.16E-34 |
| Tb927.11.2910 | Phosphoglycerate mutase, iPGAM̂ | Mitochondrion (75%), kinetoplast (75%) | Mitochondrial* | 23 | 6.79E-05 | |
| Tb927.11.7475 | AN1-like zinc finger-containing protein̂ | Cytoplasm | Zinc finger | 20 | 1.50E-30 | |
| Tb927.9.5220 | Conserved protein | Endocytic, cytoplasm (weak) | Endocytic+ | 19 | 2.60E-21 | |
| Tb927.3.4930 | Hypothetical protein, conserved | Cytoplasm, flagellar cytoplasm, | Flagellar + | 19 | 3.79E-27 | |
| Tb927.8.1930 | Isy1-like splicing family | Nucleoplasm | 9250687 | Gene Expression# | 16 | 8.36E-27 |
| Tb927.10.6850 | Mitochondrial ribosomal protein S18̂ | Cytoplasm (reticulated) | 18951088 | Mitochondrial# | 16 | 3.68E-10 |
| Tb927.11.1810 | Ring finger domain-containing protein̂ | Flagellar pocket (ring) | Flagellar + | 16 | 1.50E-18 | |
| Tb927.5.2620 | Hypothetical protein, conserved | Cytoplasm (points) | 24945722 | Gene Expression | 15 | 1.78E-09 |
| Tb927.10.390 | DUF2407 ubiquitin-like domain-containing protein̂ | Flagellum matrix proteome (BSF) | 24741115 | Flagellar* | 14 | 2.78E-12 |
| Tb927.3.1610 | CMGC/CLK family protein kinasê | Nucleoplasm, cytoplasm | 24453978 | Kinase | 14 | 2.82E-35 |
| Tb927.11.2350 | Hypothetical protein, conserved | Nucleus, cytoplasm (reticulated) | Nuclear+ | 13 | 2.19E-05 | |
| Tb927.8.7790 | Zinc finger domain, LSD1 subclasŝ | Tagging not successful, ND | Mitochondrial & ER○ | 13 | 7.88E-22 | |
| Tb927.5.4150 | Hypothetical protein, conserved | Paraflagellar rod | Flagellar* | 13 | 6.29E-06 | |
| Tb927.8.3340 | Hypothetical protein, conserved | Nucleoplasm | Nuclear+ | 13 | 1.28E-11 | |
| Tb927.10.1490 | Temperature-dependent protein affecting M2 dsRNA replication̂ | Cytoplasm (weak) | 20592024 | Gene Expression# | 12 | 6.62E-16 |
| Tb927.4.890 | Small nuclear ribonucleoprotein SmD3, putative, SmD3 | Nucleoplasm | 10900267 | Gene Expression# | 11 | 4.78E-11 |
| Tb927.7.5360 | Haemolysin-III related̂ | Cytoplasm | Pathogenesis | 10 | 8.31E-27 | |
| Tb927.7.710 | Heat shock 70-kDa protein, HSP70̂ | Cell tip (anterior), cytoplasm, flagellar cytoplasm | 30506377 | Flagellar * | 10 | 3.15E-26 |
| Tb927.9.4930 | Divalent cation transporter̂ | Cytoplasm (reticulated) | Trafficking | 9 | 7.16E-54 | |
| Tb927.9.10850 | Splicing factor 3B subunit 10, SF3b10̂ | Nucleoplasm | Gene Expression | 9 | 1.80E-49 | |
| Tb927.11.5600 | Archaic translocase of outer membrane 14-kDa subunit, ATOM14 | Mitochondrion | 22267727 | Mitochondrial# | 8 | 9.21E-11 |
| Tb927.10.9060 | Hypothetical protein, conserved | Basal body | Flagellar + | 8 | 5.86E-10 | |
| Tb927.1.1020 | Leucine-rich repeat-containing protein | Hook complex | Flagellar + | 8 | 1.61E-10 | |
| Tb927.2.2130 | Small GTP-binding protein RAB6̂ | Golgi apparatus | Golgi+ | 8 | 7.70E-22 | |
| Tb927.1.3310 | Hypothetical protein, conserved | Plasma membrane (posterior) | 18242729 | Glycosomal# | 8 | 2.28E-09 |
| Tb927.8.4200 | Hypothetical protein, conserved | Cytoplasm | 24945722 | Gene Expression# | 7 | 7.02E-19 |
| Tb927.3.5190 | Hypothetical protein, conserved | Mitochondrion | Mitochondrial+ | 7 | 8.48E-21 | |
| Tb927.9.3480 | U5Cwc21 small nuclear ribonucleoprotein | Nucleoplasm | 19429779 | Gene Expression# | 7 | 8.83E-10 |
| Tb927.8.2391 | Hypothetical protein, conserved | Not in the genome version used for tagging | ND | 7 | 9.18E-10 | |
| Tb927.2.3780 | Translation initiation factor IF-2̂ | Cytoplasm | Gene Expression | 6 | 1.12E-11 | |
| Tb927.1.1500 | Conserved protein, unknown function | Cytoplasm (reticulated) | 26784394 | Gene Expression# | 6 | 1.34E-05 |
| Tb927.6.3980 | Hypothetical protein, conserved | Axoneme [50%], cytoplasm | Flagellar + | 6 | 3.88E-10 |
a
Tryptag descriptions containing the word “putative” have been replaced with ^. Category data sources: +, TrypTag; ○, proteome; *, both; #, published.