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. 2020 Jun 9;69(11):2357–2369. doi: 10.1007/s00262-020-02622-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Intrasplenic CD11b-positive cells show increase IL-4Rα expression a May-Giemsa staining of CD11b-positive splenic cells that were isolated at 14 days and 28 days after cell implantation. Magnification, × 400. The scale bar indicates 50 μm. b Flow cytometry analysis of Gr-1 and IL-4Rα in splenic CD11b-positive cells at 14 days and 28 days after cell implantation. c The CD8 inhibitory function assay of splenic CD11b-positive cells from 28 days after cell implantation. CFSE-labeled CD8 cells stimulated with ConA were co-cultured with splenic CD11b-positive cells obtained at 28 days after implantation for 24 h. d The activated CD8 T cell population changes. Splenic cells unstimulated or stimulated by CD3 and CD28 were co-cultured with intrasplenic CD11b+ cells from 14 or 28 days after cell implantation for 24 h. − and + indicate unstimulated splenic cells and CD3/CD28 stimulated splenic cells from normal mice, respectively. *P < 0.05 and **P < 0.01 (Tukey–Kramer test). Representative data from three independent experiments that were repeated three times. One mouse per group was used 14 and 28 days after tumor implantation. One spleen of a normal mouse was used once, and the assay was repeated three times (d)

Fig. 3 — Intrasplenic CD11b-positive cells show increase IL-4Rα expression a May-Giemsa staining of CD11b-positive splenic cells that were isolated at 14 days and 28 days after cell implantation. Magnification, × 400. The scale bar indicates 50 μm. b Flow cytometry analysis of Gr-1 and IL-4Rα in splenic CD11b-positive cells at 14 days and 28 days after cell implantation. c The CD8 inhibitory function assay of splenic CD11b-positive cells from 28 days after cell implantation. CFSE-labeled CD8 cells stimulated with ConA were co-cultured with splenic CD11b-positive cells obtained at 28 days after implantation for 24 h. d The activated CD8 T cell population changes. Splenic cells unstimulated or stimulated by CD3 and CD28 were co-cultured with intrasplenic CD11b+ cells from 14 or 28 days after cell implantation for 24 h. − and + indicate unstimulated splenic cells and CD3/CD28 stimulated splenic cells from normal mice, respectively. *P < 0.05 and **P < 0.01 (Tukey–Kramer test). Representative data from three independent experiments that were repeated three times. One mouse per group was used 14 and 28 days after tumor implantation. One spleen of a normal mouse was used once, and the assay was repeated three times (d)