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. 2020 May 29;69(11):2193–2207. doi: 10.1007/s00262-020-02623-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — In vitro differentiation of M1 and M2 macrophages. a Human monocyte-derived macrophages were differentiated to M1 and M2 phenotype in the presence of LPS (50 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml), IL-10 (20 ng/ml) and TGFß (20 ng/ml), respectively. The cell surface expressions of CD80, CD206 and CD209 were measured on the two cell types by flow cytometry. Representative images of five independent experiments are shown. b, c The IL-12 and IL-10 production was measured by ELISA. The figure shows the average of three independent experiments