Fig. 2. Effect of amitriptyline on protein accumulation in mice brain.

a Mice were injected with amitriptyline (5, 10, and 20 mg/kg, i.p., n = 5) for 30 d, and immunohistochemistry against anti-ubiquitin, anti-p62, and anti-α-synuclein was performed in the cortex, striatum, and SN of mice brain. Immunoreactivities were quantified by densitometric analysis. Scale bar corresponds to 100 μm. b Motor dysfunction was assessed by the rotarod test. The latency to fall from a rotating rod was automatically recorded (n = 4–5). *P < 0.05, **P < 0.01, ***<0.001, ****P < 0.0001. AMI amitriptyline, SN subsantia nigra, Ub ubiquitin.