Fig. 3.

Keratinocytes, fibroblasts, and melanoma cells produce IL-10 mRNA in the melanoma reconstructed human skin (Mel-RhS). a IL-10 mRNA was detected at single-cell level by fluorescent RNAish in the reconstructed human skin (RhS) and Mel-RhS model. Each dot (green) indicates one IL-10 mRNA molecule. b IL-10 fluorescent RNAish (cyan) was combined with Melan-A (red; melanocytes and melanoma cells), cytokeratin (green; keratinocytes), and DAPI (blue; nuclei of all cells, including fibroblasts) immunofluorescence staining. IL-10 mRNA spots in nuclei of keratinocytes (green) and melanoma cells (red) are clearly detectable, as well as in (typically elliptical) nuclei of unstained dermal fibroblasts, as indicated by white arrows. Scale bar = 20 µm