Fig. 5.

High-dimensional analysis of the phenotype of monocytes conditioned by supernatants derived from the melanoma reconstructed human skin (Mel-RhS) model cultured in the presence or absence of IL-10 neutralizing antibodies. a Differences in the t-SNE analyses between IgG1 and anti-IL-10 conditions. Two gates with shifting subsets between conditions are shown with the percentage of total CD45⁺ monocytes in that particular gate. b Differences between IgG1 and anti-IL-10 in the intensity and the distribution of expression of CD14, BDCA3, PD-L1, CD163, and CD16 in the t-SNE analysis. The same gates as in a are depicted in b. c Percentage of M2-like cells (defined as CD14⁺BDCA3⁺CD163⁺CD16⁺PD-L1⁺PD-L2⁺) within the CD45⁺ cell population after incubation with Mel-RhS supernatant pre-treated with either IgG1 or anti-IL-10 (N = 3; mean ± SEM is shown)