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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Dev Biol. 2019 Nov 23;458(2):246–256. doi: 10.1016/j.ydbio.2019.11.010

Fig. 7. SIX2 binds the PAX9 promoter to directly promote PAX9 expression.

Fig. 7.

A. A schematic of the PAX9 promoter highlighting a consensus SIX2 binding site and a negative control (NC) region lacking a consensus SIX2 binding site. B. To test the identified binding site, ChIP was performed using either an antibody specific for SIX2 or a non-specific IgG in HEK293t cells. Primers flanking the SIX2 binding site were used to successfully amplify a product from input chromatin used in ChIP reactions as well as from chromatin isolated from SIX2 ChIP, but failed to amplify a product from the IgG reaction. C. Primers flanking an NC region of the PAX9 promoter not containing a SIX2 binding site amplified products from input samples but not from ChIP reactions. D. SIX2 and PAX9 expression were assayed by Western blot in HEPM cells overexpressing empty vector, SIX2, or SIX2 (p.Gly264Glu).