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An Initial Gate (A) is set on a CD45-ECD vs SSC dot plot ,so as to contain all CD45dim and CD45 bright .This exclude CD45 negative events(i.e,red cells, platelets and other debris) Fig 2B :CD34+-PE vs HLADR-FITC dot plot and event in gate A1 shows 21.9% of positive CD34+ cells Gate A4 shows 6.7% of HLADR positive cells white gate A2 displayed 3.6% of both CD34+ and HLADR positive cells
