Figure 1.
RT-qPCR Analyses of EGFR mRNA in U373-MG Cells. To measure the expression of EGFR in glioblastoma cells, the U373-MG cells were transfected with negative control (NC) miRNA and miRNA-7 for 24 and 48 h. Relative EGFR mRNA expression was quantified by RT-qPCR using 2 - (∆∆Ct) method and β-actin as an internal control. Data are presented as mean ± SD of three independent experiments. *p < 0.05; #p > 0.05 versus blank control