Effect of EIDD-2801 treatment on viral clearance and adaptive antiviral response. A) Schematic of the experimental setup. B) Shed virus titer in nasal lavages, taken in 12-hour intervals. C) Inflammatory cell count in nasal lavages from (B). D) Hemagglutination inhibition (HI) serum antibody titers in vehicle and EIDD-2801-treated animals, determined from blood samples taken on day 0, 3, 7, 10, and 14 after infection. Serum samples were tested against the Ca/09 strain. Symbols represent biological repeats (N = 3), lines connect medians. Statistical significance was determined by 2-way ANOVA with Sidak’s multiple comparisons post-hoc test.