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. 2020 Oct 14;12:10151–10161. doi: 10.2147/CMAR.S263768

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© 2020 Wang et al.

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Hypoxic NSCLC cell-derived exos transmit miR-582-3p to normoxic NSCLC cells. (A) miR-582-3p mimics transfection markedly up-regulated the levels of miR-582-3p in hypoxic A549 cells. (B) The levels of miR-528-3p in miR-582-3p-exos and NC-exos was analyzed using qRT-PCR assay. (C) Western blot analysis of the enrichment of CD63, CD9 and Alix in exos purified from hypoxic A549 cell line transfected with miR-582-3p mimic (miR-582-3p-exos) or NC (NC-exos). (D) The concentration and size distribution of exos were detected by nanosight particle tracking analysis. (E) The exo uptake assay was conducted to confirm the uptake of PKH26-labeled miR-582-3p-exos (red) into recipient normoxic A549 cells. Scale bar: 20 μm. (F) Both NC-exos or miR-582-3p-exos treatment significantly increased the levels of miR-582-3p in A549 and H1299 cells. The experiments were repeated three times. Data were expressed as mean±standard deviation. **p<0.01 and ***p<0.001.