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. 2020 Oct 14;12:10151–10161. doi: 10.2147/CMAR.S263768

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© 2020 Wang et al.

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Hypoxic NSCLC cell-secreted exosomal miR-582-3p abrogates the effect of SFRP1 on the malignant phenotypes of normoxic NSCLC cells. A549 and H1299 cells were transfected with SFRP1-oe. and then treated with miR-582-3p-exos. (A) The protein levels of SFRP1 were analyzed using Western blot assay. (B) The viability of A549 and H1299 cells was analyzed by CCK-8 assay. The migration and invasion abilities of A549 and H1299 cells were analyzed using wound healing assay (C) and transwell assay (D). The experiments were repeated three times. Data were expressed as mean±standard deviation. *p<0.05, **p<0.01, and ***p<0.001.