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. 2020 Oct 5;11:559590. doi: 10.3389/fimmu.2020.559590

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Copyright © 2020 Cotugno, Santilli, Pascucci, Manno, De Armas, Pallikkuth, Deodati, Amodio, Zangari, Zicari, Ruggiero, Fortin, Bromley, Pahwa, Rossi, Pahwa and Palma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design. The cartoon on the top panel depicts the experimental procedure. Briefly, total PBMCs are in 2 aliquotes, one stimulated and the latter unstimulated. After sorting lymphocite subsets, gene expression is analyzed by Fluidigm Biomark. Bottom panel describes the gating strategies and the lymphocites subsets selected for sorting and gene expression analysis. Mathematical analysis applied on the subsets in order to obtain differentially expressed genes (DEGs) and differentially induced genes (DIGs) are described.