FIG 6.

Restriction of SARS-CoV-2 by transient ZAP-L and ZAP-S expression. (A) Quantification of viral N gene RNA copies by qRT-PCR in the supernatant of ZAP KO HEK293T cells cotransfected with an ACE2 expression vector and increasing concentrations of vectors expressing ZAP-L or ZAP-S. Viral RNA yield was determined 48 h postinfection with SARS-CoV-2. Shown are mean values (±SD) obtained in three independent experiments, each measured in duplicate. Stars indicate significant (P value) differences from the no-ZAP control results as follows: *, <0.05; **, <0.01; ***, <0.001.The right panel shows a Western blot of whole-cell lysates stained with anti-ACE2, anti-ZAP-L and anti-ZAP-S, anti-SARS-CoV-2 Spike, and anti-GAPDH as loading control. (B) Schematic presentation of the SARS-CoV-2 genome and the positions of the primer binding sites in the full-length or subgenomic viral RNAs. (C) Effect of ZAP-L and ZAP-S on the levels of the indicated viral RNAs. Shown are mean values (±SD) from two independent experiments relative to the RNA levels obtained after cotransfection of ZAP-L or ZAP-S expression vectors compared to empty control vector (100%).