Figure 5.

miR-31-5p in MSC-Exosomes Inhibited Apoptosis and Calcification in EPCs under Oxidative Stress

(A) Impact of miR-31-5p on EPC apoptosis was examined using the flow cytometry assay. (B) Agomir-31-5p significantly alleviated EPC apoptosis, whereas the antagomir-31-5p exacerbated apoptosis. (C) Impact of miR-31-5p on EPC calcification was evaluated using alizarin red staining, ALP staining (scale bars, 50 μm), and immunofluorescence staining of Runx2 (scale bars, 200 μm). (D) Agomir-31-5p significantly alleviated EPC calcification, whereas antagomir-31-5p exacerbated calcification. (E) Anti-apoptotic activities of miR-31-5p-depleted MSC-exosomes were evaluated using flow cytometry. (F) The percentage of the apoptotic cells was increased after silencing miR-31-5p in MSC-exosomes. (G) Anti-calcification activities of miR-31-5p-depleted MSC-exosomes were detected using alizarin red staining, ALP staining (scale bars, 50 μm), and immunofluorescence staining of Runx2 (scale bars, 200 μm). (H) The percentage of the Runx2-positive cell was increased after silencing miR-31-5p in MSC-exosomes. Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.