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. 2020 Oct 16;40(10):BSR20201532. doi: 10.1042/BSR20201532

Figure 3. Relationships among barrier parameters for differentiated 16HBE cell layers.

Figure 3

(A) Short-circuit current dependence on temperature; (B) TER dependence on temperature; (C) TER dependence on short-circuit current; (D) lack of relationship between TER and 14C-mannitol flux rates for already formed 16HBE barriers. Seeded at 2.0 × 106 cells per 4.2 cm2 PCF on day 0, TER and Isc were determined as described in ‘Materials and methods’ section on day 7 post-seeding for n=8 cell layers (A,C) or n=16 cell layers (B) recorded at varying temperatures. For panel (D), n=57 cell layers treated under control conditions throughout various experiments were seeded at 1.0 × 106 or 2.0 × 106 cells per PCF on day 0, TER and Jm were determined as described in ‘Materials and methods’ section on days 6, 7, and 8 post-seeding.