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. 2020 Oct 16;40(10):BSR20201532. doi: 10.1042/BSR20201532

Figure 6. Effect of 24-h exposure to TNF-α (A) or TPA (B) on 16HBE tight junctional proteins.

Figure 6

Cell layers used in the barrier function studies from Figure 5 were harvested from filters, and the total cell lysates were analyzed for tight junctional proteins in Western immunoblots as described in ‘Materials and methods’ section. Data are represented as mean ± standard error for n=4 cell layers for each condition. ** indicates P<0.01, *** indicates P<0.001, N/S indicates not significant (treatment compared with control for that experiment, Student’s t test, two-tailed). ‘C’ indicates control conditions.