Table 2. Diffusion potential values across 16HBE cell layers.
| ΔV10 (mV) | TER1 (Ohms × cm2) | TER2 (ohms × cm2) | PCl/PNa | |
|---|---|---|---|---|
| Mean ± SEM | −9.15 ± 0.4 | 780 ± 59 | 763 ± 57 | 1.91 ± 0.06 |
Diffusion potential measurements were performed as described in ‘Materials and methods’ section for n=4 cell layers. PCFs were seeded at 2.0 × 106 cells at day 0 and the experiment was performed at day 7 post-seeding. ΔV10 represents the potential difference (apical negative) across 16HBE with an apically directed 100 mM NaC1 gradient at t = 10 min. TER1 represents the baseline TER value recorded in culture medium prior to the experiment and TER2 represents the TER value in Saline A after the experiment. PCl/PNa represents the relative permeability of the 16HBE cell layer to chloride vs sodium with the imposed 100 mM NaC1 gradient. (See Supplemental File regarding liquid junction potential measurements)