Figure 3.

Mobilization of crLV-Derived NIH45-46 CAR

(A) crLV vectors are capable of functioning only in cells that are infected with HIV (1) that is actively reverse transcribed (2), integrated (3), replicating (5), packaged (7), and released (8), whereby they will hijack the machinery to package the crLV-derived CAR into the virion. The crLV-derived CAR will use the viral machinery to be transcribed (6), packaged (7), and released into the virion (9). (B) Flow cytometric analysis of one of the NL4-3 mobilization assays. Cells were collected on days 4 and 7 after supernatant transfer and stained for EGFR. (C) Graphical analysis of mobilization of crLV-derived CAR using two different X4-tropic viruses at day 7. LAI data are the representation of two experiments, and NL4-3 is the representation of four experiments.