Fig. 4.

Correlation of CYP2D6 protein abundance and the rate of biotransformation of pimozide to 5-hydroxypimozide (5-OH-Pimozide) (left) and 6-hydroxypimozide (6-OH-Pimozide) (right) in HLMs isolated from individual donors (n = 7). Data points represent the means of separate experiments (n = 3), and error bars reflect the S.D.; error bars are not visible for many points because of low interexperimental variability. The r² corresponding to the fit of a simple linear regression model for each metabolite is listed at the top of each respective graph. Expression of CYP2D6 protein was determined via MS/MS analysis. Data points were color coded to reflect activity score assignments for CYP2D6 genotypes: 0 (red), 0.5 (yellow), 1.0 (green), 2.0 (blue), and >2.25 (purple) (Caudle et al., 2020).