Fig. 4.
Effects of TRPA1 or TRPV3 inhibition on pine WSPM–induced ERS biomarker induction. Expression of ATF3 (A), DDIT3 (B), HSPA1A (C), and spliced XBP1 (D) mRNA 24 hours after treatment of lobar HBECs with pine WSPM (20 μg/cm2) or carvacrol (250 μM), in the presence of either the TRPA1 antagonist A967079 (20 μM) or the TRPV3 antagonist (TRPV3 Ant.; 10 μM). Data were normalized to the vehicle-treated group and are represented as means ± S.D. from n = 3 replicates. Statistical significance was determined using an ordinary two-way ANOVA using the Tukey post-test comparing all treatments for each group. **P < 0.01; ****P < 0.0001 relative to the respective no antagonist control. ###P < 0.001; ####P < 0.0001 indicate differences between the TRPA1 and TRPV3 antagonist groups.