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. 2020 Nov;98(5):586–597. doi: 10.1124/molpharm.120.000047

Fig. 7.

Fig. 7.

Effects of shRNA-driven TRPV3 knockdown on the sensitivity of BEAS-2B cells to pine WSPM–induced ERS. mRNA expression of TRPV3 (A and C) and DDIT3 (B and D) in BEAS-2B cells stably overexpressing control/scrambled shRNA (shScramble; white bars) or shRNA targeting TRPV3 (shTRPV3; black bars) after 4-hour treatment with vehicle and pine WSPM (20 μg/cm2) (A and B) or 20-hour treatment with vehicle and pine WSPM (10 μg/cm2) (C and D). Data were normalized to the vehicle-treated shTRPV3 cells and are presented as means ± S.D. from n = 3 replicates. **P < 0.01; ***P < 0.001; ****P < 0.0001 compared with vehicle treated control cells, #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001 compared with pine WSPM treated control cells, and ^P < 0.05; ^^^^P < 0.0001 compared with vehicle-treated scramble control cells using an ordinary two-way ANOVA and the Tukey post-test comparing all data sets.