TABLE 1.
Effects of TRPV3 overexpression on BEAS-2B cell responses to pine WSPM and prototypical ERS-inducing agents
Relative expression of mRNA for ERS biomarkers in BEAS-2B (B2B) and TRPV3OE cells, after 4-hour treatment with various ERS-inducing agents. Data are presented as relative mRNA expression, normalized to vehicle-treated BEAS-2B cells and are represented as means ± S.D. from n = 3 replicates.
| Treatment | ATF3 | DDIT3 | HSPA1A | XBP1s/XBP1u | ||||
|---|---|---|---|---|---|---|---|---|
| BEAS-2B | TRPV3OE | BEAS-2B | TRPV3OE | BEAS-2B | TRPV3OE | BEAS-2B | TRPV3OE | |
| Vehicle | 1.0 ± 0.3 | 1.2 ± 0.4 | 1.0 ± 0.2 | 1.2 ± 0.2 | 1.0 ± 0.0 | 0.4 ± 0.0 | 1.0 ± 0.2 | 0.8 ± 0.2 |
| Pine WSPM (1 μg/cm2) | 2.2 ± 0.7 | 3.4 ± 0.4 | 4.3 ± 0.7 | 2.0 ± 0.3a | 4.4 ± 0.3 | 0.9 ± 0.3a | 2.8 ± 0.5 | 1.3 ± 0.1 |
| Pine WSPM (5 μg/cm2) | 6.1 ± 0.9 | 12 ± 1a | 12 ± 2 | 3.9 ± 0.5a | 24 ± 2 | 7 ± 2a | 2.4 ± 0.6 | 0.9 ± 0.1 |
| Pine WSPM (20 μg/cm2) | 40 ± 4 | 7 ± 1a | 17 ± 2 | 4 ± 1a | 16.0 ± 0.2 | 1.7 ± 0.2a | 9 ± 2 | 1.0 ± 0.1 |
| Thapsigargin (10 μM) | 95 ± 7 | 33 ± 13a | 46 ± 2 | 33 ± 7 | 0.1 ± 0.0 | 0.1 ± 0.0 | 18.2 ± 0.9 | 8 ± 2a |
| DTT (250 μM) | 2.4 ± 0.2 | 12 ± 1a | 15.0 ± 0.2 | 10.2 ± 0.8a | 0.2 ± 0.3 | 0.4 ± 0.3 | 4.9 ± 0.1 | 2.9 ± 0.2a |
| Eeyarestatin I (4 μM) | 15 ± 2 | 18 ± 3 | 21 ± 2 | 15 ± 1a | 0.9 ± 0.6 | 2.7 ± 0.6 | 3.1 ± 0.5 | 1.9 ± 0.1 |
| Tunicamycin (2.5 μg/ml) | 12 ± 2 | 17 ± 2 | 59 ± 8 | 41 ± 3 | 0.1 ± 0.0 | 0.1 ± 0.0 | 22 ± 2 | 12 ± 3 |
| H2O2 (1 mM) | 4.7 ± 0.4 | 1.1 ± 0.2a | 10.5 ± 0.9 | 4.0 ± 0.6a | 0.1 ± 0.0 | 0.4 ± 0.0 | 1.3 ± 0.3 | 1.0 ± 0.2 |
| 2-Deoxy-d-glucose (10 mM) | 25 ± 4 | 11.7 ± 0.5a | 49 ± 6 | 22 ± 2a | 0.3 ± 0.0 | 0.1 ± 0.0 | 9 ± 3 | 3.6 ± 0.3 |
DTT, dithiothreitol.
a
Indicates statistical significance between BEAS-2B and TRPV3OE cells determined using multiple t tests for each gene and a false discovery rate of 5%.