Fig. 1.

Female and male brainstem microglia share similar transcriptomes. (A) Principle component analysis of male and female brainstem (BS) microglia (n = 5/treatment). (B) Venn diagram showing the total numbers of shared and unique genes between males and females. There were 13,517 shared genes that did not significantly differ in either sex. There were 28 differentially expressed genes that significantly differed (FDR < 0.05) between females and males. Upregulated genes are shown in red, and downregulated genes are shown in blue. (C) Significantly upregulated genes (12) in female brainstem microglia vs. males. Genes highlighted in pink are X chromosome–linked. (D) Significantly downregulated genes (16) in female brainstem microglia vs. males. Genes highlighted in pink are X chromosome–linked, and those in blue are Y chromosome–linked.