Fig. 2.

PMA stimulated SAOS2 cells to secrete and release VWF. (A) SAOS2 and MG63 cell lysates were used in immunoblotting assay. HMEC-1 and platelet lysates were used as positive controls. HEK293 was used as a negative control; (B) immunofluorescence staining detected VWF expression level in SAOS2 cells treated with or without PMA. Hoechst 33342 staining (blue) marked the nucleus. VWF (green) was stained with FITC-conjugated anti-VWF polyAb; (C) SAOS2 and MG63 cell treated with or without PMA were used in immunoblotting assay; (D) flow cytometric analyses of VWF expressed on the surface of SAOS2 cells treated with or without PMA, MG63 was used as a negative control; (E) VWF multimeric structure in SAOS2 and HMEC-1 cells treated with or without PMA, human plasma and MG63 cells was analyzed by agarose gel electrophoresis. Separate bands were visualized by chemiluminescence to show various sizes of VWF molecular. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)