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. 2020 Oct 8;25:100325. doi: 10.1016/j.jbo.2020.100325

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3 — Inhibition effect of VWF-GPIb pathway blocking antibodies in platelets adhesion assay. (A) Platelets adhesion assay was performed on PMA-/NS, PMA+/Mouse IgG, PMA+/SZ123, PMA+/SZ2 and PMA+/AVW3. Platelets (green) were treated with staining reagent Calcein-AM. SAOS2 cells (blue) were labeled with cytoplasmic staining reagent Hoechst 33342. VWF (red) was stained with mAb SZ-34 for visualization; (B) quantification of VWF expressed on the surface of SAOS2 cells in PMA-/NS, PMA+/Mouse IgG, PMA+/SZ123, PMA+/SZ2 and PMA+/AVW3. ***P < 0.001; (C) quantification of surface covered by adherent platelets to SAOS2 cells in PMA-/NS, PMA+/Mouse IgG, PMA+/SZ123, PMA+/SZ2 and PMA+/AVW3. ***P < 0.001. Mean ± SD is shown for three separate experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)