Fig. 4.

Inhibition effect of VWF-GPIb pathway blocking antibodies in platelets adhesion assay under shear stress. (A) the microscopic images of heparinized whole blood stained with Calcein-AM (green) perfused over SAOS2 cells at a constant high shear rate of 1000/s for 15 min in PMA-/NS, PMA+/Mouse IgG, PMA+/SZ123, PMA+/SZ2 and PMA+/AVW3. ***P < 0.001; (B)–(E) quantification of surface covered by adherent platelets to SAOS2 cells in PMA-/NS, PMA+/Mouse IgG, PMA+/SZ123, PMA+/SZ2 and PMA+/AVW3 at 2, 5, 10 and 15 min. ***P < 0.001. Mean ± SD is shown for three separate experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)