Fig. 5.

Inhibition effect of VWF-GPIb pathway blocking antibodies in transmigration and invasion capacity of SAOS2 cells treated with PMA. (A) the microscopic images of SAOS2 and MG63 (negative control) cells treated with PMA and stained with CFSE (green) that transmigrated through the membrane barrier in Cell, Cell + PLT, Cell + PLT + SZ123, Cell + PLT + SZ2 and Cell + PLT + AVW3; (B) the microscopic images of SAOS2 and MG63 (negative control) cells treated with PMA and stained with CFSE (green) that invaded through the matrigel membrane barrier in Cell, Cell + PLT, Cell + PLT + SZ123, Cell + PLT + SZ2 and Cell + PLT + AVW3; (C, D) quantification of transmigrated and invaded cells. Mean ± SD is shown for three separate experiments. **P < 0.01, ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)