Table 2.
Peripheral blood mononuclear cell subsets
| Markers | Cell subset | Controls (n=28) |
DLB (n=27) |
AD (n=26) |
Test statistic P value |
|
| CD3+ | CD3+ | T cells | 64.2 53.4–72.0 |
62.6 57.2–71.3 |
59.1 55.1–65.2 |
1.287* 0.526 |
| CD4+ | CD3+ CD4+ | Helper T cells | 57.9±16.7 | 49.6±19.5β | 61.1±14.9 |
3.209† 0.046 |
| HLA-DR+ MFI | Activated helper T cells | 10 246 6866–14950 |
10 128 7730–13491 |
9843 8354–12019 |
0.247* 0.884 |
|
| CD45RA+CCR7+ | Naïve helper T cells | 62.2 44.7–75.0 |
60.4 41.9–70.5 |
65.4 47.6–79.7 |
1.760* 0.415 |
|
| CD45RA-CCR7+ | Central memory helper T cells | 18.5±8.1 | 20.6±9.3 | 19.5±10.8 | 0.343† 0.710 |
|
| CD45RA-CCR7- | Effector memory helper T cells | 12.6 6.9–24.4 |
11.3 8.1–22.7 |
12.4 6.4–19.0 |
1.134* 0.567 |
|
| CD45RA+CCR7- | Terminal effector helper T cells | 1.8 1.1–5.6 |
2.5 1.3–5.6 |
1.8 0.8–3.7 |
0.716* 0.699 |
|
| CD8+ | CD3+CD8+ | Cytotoxic T cells | 29.9 22.5–35.1 |
40.1 26.6–53.9 |
25.0 20.3–37.0 |
5.868* 0.053 |
| HLA-DR+MFI | Activated cytotoxic T cells | 5321 4652–6712 |
5530 4244–6980 |
5879 4281–8076 |
0.435* 0.804 |
|
| CD45RA+CCR7+ | Naïve cytotoxic T cells | 29.4 13.1–40.4 |
21.8 13.1–31.2 |
28.1 13.7–48.1 |
1.188* 0.552 |
|
| CD45RA-CCR7+ | Central memory cytotoxic T cells | 5.3 2.9–8.0 |
4.6 2.4–6.3 |
5.4 2.0–7.5 |
0.213* 0.899 |
|
| CD45RA-CCR7- | Effector memory cytotoxic T cells | 22.4 12.0–31.6 |
19.0 7.2–28.2 |
16.7 8.8–25.4 |
2.042* 0.360 |
|
| CD45RA+CCR7- | Terminal effector cytotoxic T cells | 42.8±20.9 | 50.8±20.0 | 45.2±20.8 | 1.095† 0.339 |
|
| CD19+ | CD3-CD19+ | B cells | 4.7 3.6–6.0 |
4.7 2.3–6.9 |
5.5 3.8–8.2 |
3.101* 0.212 |
| HLA-DR+MFI | Activated B cells | 61861±14 832 | 53442±1672β 9 | 66530±15 324 |
4.786† 0.011 |
|
| CD14+ | CD3-CD14+ | Monocytes | 15.0 8.5–20.2 |
15.2 10.6–22.5 |
17.0 11.9–23.0 |
1.392* 0.499 |
Results are presented as mean±SD or median with LQ-UQ below, and as percentage of parent population, or (for human leucocyte antigen-antigen D related) mean fluorescent intensity in standard units. Statistical tests are denoted with symbols.
Post-hoc results: α=significantly different from controls (p<0.05), β=significantly different from AD (p<0.05).
*Kruskal-Wallis.
†Significantly different from AD (p<0.05).
‡χ2.
CCR7, C-C chemokine receptor type 7; CD, cluster of differentiation; HLA-DR, Human leukocyte antigen – antigen D related; MFI, mean fluorescent intensity.