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. 2020 Sep 17;75(11):1004–1008. doi: 10.1136/thoraxjnl-2020-215027

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Patient-derived malignant pleural mesothelioma (MPM) cell cultures originated from malignant pleural effusion specimens are truly cancerous and showed tumour stemness properties. (A–E) Top: phase contrast images (10× magnification) of representative MPM cells in culture showing colony formation (white arrows), cobblestone (black arrows) and spindle (red arrows) shapes. Bottom: May Grunwald Giemsa-stained cytospin specimens of selected MPM cell cultures showing (A) pleomorphic and multiple nucleoli (10× magnification), (B) small atypical nucleoli and two-tone cytoplasm typical of mesothelial morphology (40× magnification), (C) atypical features with large nucleus and very large nucleoli (40× magnification), (D) bizarre nucleus with multiple nucleoli (40× magnification), (E) large and multiple nuclei (binucleate) and atypical and multiple nucleoli (40× magnification). (F–M) Phase contrast images of tumour-spheroids formed by MPM patient-derived cancer cell cultures (10× magnification). Patient-derived MPM cell cultures were able to form tumour-spheres highlighting tumour stemness properties and the existence of a cancer stem cell subpopulation.