Figure 4.

T-TNF regulates memory T cell responses during CIA. (A) Analysis of TNF production in inflamed paws in TNF KO, T-TNF KO, M-TNF KO and WT mice at day 14 after second immunisation. (B, C) Frequency of anticollagen type II Th1 (IFNγ⁺) and Th17 (IL-17A⁺) CD4 T cell responses in T-TNF KO, M-TNF KO and WT mice in spleen at day 14 after second immunisation. Frequency of total IL-12p40-producing cells (D), IL-12p40-producing inflammatory monocytes (CD11b⁺Ly6C⁺Gr1^low) and (CD11c⁺MHCII⁺) (E) in spleens of WT, T-TNF KO and M-TNF KO mice at day 6 after immunisation. Splenocytes were stimulated with heat-killed M.TB H37Ra (100 mcg/mL) for 6 hours in the presence of brefeldin A and were analysed for the IL-12p40 expression using flow cytometry. All data are representative of two or more independent experiments with n≥3. *p<0.05, **p<0.01, ***p<0.001, as calculated by Kruskal-Wallis non-parametric test with Dunn's multiple comparisons test. CIA, collagen-induced arthritis; M-TNF, mice lacking TNF in myeloid cells; T-TNF, TNF ablation in T lymphocytes; TNF, tumour necrosis factor; WT, wild-type.