Reagent or resource	Source	Identifier
Antibodies
Anti‐ERK1/2, Rabbit	CST	9102
Anti‐ERK1/2, Mouse	CST	4696
Anti‐phospho‐ERK1/2(Y202/Y204), Rabbit	CST	4370
Anti‐MEK1/2, Mouse	CST	4694
Anti‐phospho‐MEK1/2(S217/S221), Rabbit	CST	9121
Anti‐CRAF, Mouse	CST	12552
Anti‐BRAF, Mouse	Invitrogen	MA5‐15495
Anit‐panRAS, Mouse	Cytoskeleton	AESA02
Anti‐phospho‐AKT(S473)	CST	4060
Anti‐beta‐Actin	Sigma‐Aldrich	A2228
Anti‐Tubulin	CST	3873
Anti‐GAPDH	CST	2118
IRDye 800CW Donkey anti‐Rabbit IgG	Licor	925‐32213
IRDye 800CW Donkey anti‐Mouse IgG	Licor	926‐32212
IRDye 680RD Donkey anti‐Rabbit IgG	Licor	926‐68073
IRDye 680RD Donkey anti‐Mouse IgG	Licor	925‐68072
Chemicals, peptides, and recombinant proteins
Amphiregulin	Peprotech	100‐55B
EGF	Peprotech	AF‐100‐15
FGF	Peprotech	100‐18B
HGF	Peprotech	100‐39
IGF‐I	Peprotech	100‐11
PDGF‐AB	Peprotech	100‐00AB
ARS‐853	MedChemExpress	HY‐19706
PD0325901	Selleck Biochemicals	S1036
SCH772984	Selleck Biochemicals	S7101
Blasticidin	Corning	30‐100‐R1
Bovine serum albumin	Sigma‐Aldrich	A7906
Collagen I, rat tail	Life Technologies	A10483‐01
Dextrose	Fisher	C6H1206
l‐Glutamine	Life Technologies	25030‐081
Puromycin dihydrochloride	Sigma‐Aldrich	P8833
DMEM	Life Technologies	11965‐092
Fetal bovine serum	Gemini Bio‐products	100‐106
Penicillin streptomycin	Life Technologies	15070‐063
0.25% Trypsin‐EDTA	Life Technologies	25200‐056
Tris Base	Fisher	BP152
Glycine (Crystalline Powder)	Fisher	BP381
Sodium dodecyl sulfate (SDS), Micropellets	Fisher	BP8200500
Ponceau S solution, suitable for electrophoresis, 0.1% (w/v) in 5% acetic acid, 1L	Sigma‐Aldrich	P7170‐1L
Bromophenol blue	Sigma‐Aldrich	B5525
Dithiothreitol	Fisher	BP172
Critical commercial assays
Amaxa MEF 2 Nucleofector kit	Lonza	VPD‐1005
Experimental models: cell lines
RAS‐less MEF (Mouse Embryonic Fibroblast) cell lines with transduced human RAS or BRAFV600E	Gift from Dom Esposito, Frederick National Laboratory
HRASWT EKAR3	This paper	Available on request
KRAS4BWT EKAR3	This paper	Available on request
NRASWT EKAR3	This paper	Available on request
KRASG12C EKAR3	This paper	Available on request
KRASG12D EKAR3	This paper	Available on request
KRASG12V EKAR3	This paper	Available on request
KRASQ61R EKAR3	This paper	Available on request
BRAFV600E EKAR3	This paper	Available on request
Recombinant DNA
Plasmid: pPBJ‐EKAR3nls‐puro	Sparta et al (2015)	Addgene # forthcoming
Software and Algorithms
NIS Elements AR ver. 4.20	Nikon	RRID:SCR_014329
Bio‐Formats ver. 5.1.1 (May 2015)	OME	RRID:SCR_000450
uTrack 2.0	Jaqaman et al (2008)	http://www.utsouthwestern.edu/labs/danuser/software/
MATLAB	Mathworks	SCR_001622
Other
Glass‐Bottom Plates, #1.5 cover glass	Cellvis	P24‐1.5H‐N, P96‐1.5H‐N
12% Mini‐PROTEAN® TGX™ Precast Protein Gels, 15‐well, 15 μl	Bio‐Rad	4561046
SuperSep Phos‐tag gels (50 μmol/l), 12.5%, 17 wells	Wako‐Chem	195‐17991
GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls, 0.1 μm pore	Fisher	45‐004‐000