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Guttiferone K has no effect on the MAPK pathway. RAW264.7 cells (a) or primary peritoneal macrophage cells (b) were infected with Mtb and treated with or without GK (10 μM) for 30, 60, or 180 min. The protein levels of JNK, p-JNK, ERK, p-ERK, p38, p-p38, and β-actin were detected by Western blot and normalized to their respective nonphosphorylated total proteins. The results are representative of at least three independent experiments. NS: not significant.
