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. 2020 Oct 10;2020:9864370. doi: 10.1155/2020/9864370

Figure 4.

Figure 4

Crocetin improves mitochondrial dysfunctions in MPP + -damaged BV2 cells. BV2 cells were treated with 0.5 mM MPP + for 6 h, and then 2.5 μM, 5 μM, and 10 μM crocetin were added and incubated for another 24 h. ((a), (b)) ROS was detected using DCFH-DA and measured by flow cytometer. (c) Mitochondrial membrane potential was measured using JC-1 dye and measured by a microplate reader. (d) Total ATP amounts in each group were measured. (e) The release of cytochrome c was measured between cytosol and isolated mitochondria. (f) The content of calcium in the cytosol was detected by Fluo-4AM and measured by a fluorescence microplate reader. Data are expressed as the mean ± standard deviation, and statistical analysis was performed using a one-way analysis of variance followed by Tukey's post hoc test. ##P < 0.01 versus control; ∗P < 0.05, ∗∗P < 0.01 versus MPP+. n = 5 in each group.