Figure 4.

AtPPRT1 reduces tissue damage caused by heat stress. (A) DAB-staining assay of 7-day-old seedlings of Col-0, atpprt1, OE2 and OE10 after heat stress. Scale bar: 1 mm; (B) detection of ROS in 7-day-old seedlings of different genotypes by using H₂DCFDA. Seedlings from Col-0, atpprt1, OE2 and OE10 were exposed to 45 °C for 3 h. Fluorescence of ROS (green) was imaged in cotyledon and root cells, and the images were collected after 15 min; (C) content of (a) H₂O₂ and (b) MDA in Col-0, atpprt1, OE2 and OE10 before and after heat stress. Error bars represent ± SD (n = 3, * p < 0.05, ** p < 0.01, t-test).