Figure 3.

Down-regulation of P. aeruginosa QS genes. RNA was isolated from P. aeruginosa cultures treated and untreated with anti-diabetic drugs in sub-MIC to be used in cDNA synthesis. The cDNA was amplified by qRT-PCR and changes in the expression of each QS gene that were normalized in relation to the mean critical threshold values of housekeeping gene rpoD. Expression fold change in gene expression in anti-diabetic treated P. aeruginosa was calculated by the 2^−ΔΔCT method and compared to untreated bacteria. The data shown are the mean ± standard errors from three experiments. p < 0.05 was considered significant using the one-way ANOVA test. Three tested drugs significantly decreased the expression of genes lasI, lasR, rhlI, rhlR, pqsA and pqsR (p < 0.0001). ***: p ≤ 0.001.